DNA

Part:BBa_K4077008:Design

Designed by: Kavya Prince, Himanshu Ranjan   Group: iGEM21_NIT_Warangal   (2021-10-17)


Laccase from Melanocarpus albomyces gene insert


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3180
    Illegal PstI site found at 959
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3888
    Illegal PstI site found at 959
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2233
    Illegal BglII site found at 2536
    Illegal XhoI site found at 1180
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3180
    Illegal PstI site found at 959
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3180
    Illegal PstI site found at 959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5228
    Illegal BsaI.rc site found at 2262


Design Notes

No special considerations.


Source

Pichia pastoris expression vector and Laccase from Melanocarpus albomyces.

References